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SUMMARY The brain exhibits remarkable neuronal diversity which is critical for its functional integrity. From the sheer number of cell types emerging from extensive transcriptional, morphological, and connectome datasets, the question arises of how the brain is capable of generating so many unique identities. ‘Terminal selectors’ are transcription factors hypothesized to determine the final identity characteristics in post-mitotic cells. Which transcription factors function as terminal selectors and the level of control they exert over different terminal characteristics are not well defined. Here, we establish a novel role for the transcription factorbroadas a terminal selector inDrosophila melanogaster. We capitalize on existing large sequencing and connectomics datasets and employ a comprehensive characterization of terminal characteristics including Perturb-seq and whole-cell electrophysiology. We find a single isoformbroad-z4serves as the switch between the identity of two visual projection neurons LPLC1 and LPLC2.Broad-z4is natively expressed in LPLC1, and is capable of transforming the transcriptome, morphology, and functional connectivity of LPLC2 cells into LPLC1 cells when perturbed. Our comprehensive work establishes a single isoform as the smallest unit underlying an identity switch, which may serve as a conserved strategy replicated across developmental programs. 

AMA1 and SPS1 control distinct aspects of meiosis II spindle disassembly, with AMA1 affecting the loss of Ase1 and Cin8 during meiosis II spindle disassembly, while SPS1 affects Bim1. The Anaphase Promoting Complex and meiotic/mitotic exit pathways seem to regulate similar targets in meiosis as mitosis, despite utilizing meiosis-specific regulators in those pathways.